Pcr Troubleshooting And Optimization The Essential Guide Book Free 19

For traditional cationic lipid reagents, we recommend testing various amounts of transfected DNA (0.25, 0.5, 0.75 and 1µg per well in a 24-well plate) at two charge ratios of lipid reagent to DNA (2:1 and 4:1; see Figure 5). This brief optimization can be performed using a transfection interval of 1 hour under serum-free conditions. One 24-well culture plate per reagent is required for the brief optimization with adherent cells (three replicates per DNA amount).

pcr troubleshooting and optimization the essential guide book free 19

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